BIOTECHNOLOGY PRINCIPLES & PROCESSES
1. Significance of heat shock method in bacterial transformation is facilitate
2.Process of formation of RNA from DNA is called
3.Consider the following statements I. Recombinant DNA technology popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic material by man in vitro II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriquez from E.coli plasmid III. Restriction enzymes belongs to a class of enzymes called nucleases Which of the statements given above are correct?
4.Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segment of DNA?
5.The transgenic animals are those which have:
6.Recombinant DNA technology is related with:
7.Enzyme that is used in PCR technology is
8.Which one of the following is commonly used in transfer of foreign DNA into crop plants?
9.A collection of organisms, usually viruses, bacteria or yeast, which have been transformed by the addition of extra genes from another species:
10.Single-stranded DNA molecules that can bind to and be used to detect other DNA molecules are called
11.The minimum length of cistron in base pairs which synthesizes a polypeptide of 50 amino acids is:
12.Which one of the following techniques had helped to solve many mysteries involving murders, robberies and rapes?
13.In agarose gel electrophoresis, DNA molecules are separated on the basis of their
14.In the naming of restriction enzymes the first letter is derived from _A_ name and next two letters from the _B_ and fourth letter from _C_ of _D_ where the enzymes are extracted A to D in the statement can be
15.Gene therapy involves:
16.Restriction enzymes may be used for:
17.Retroviruses in animals including humans are able to change normal cells into
18.Genetic diagnosis by DNA testing:
19.$T_1$ plasmid is used for making transgenic plants. It is obtained from:
20.Find the incorrect statement:
21.In case of polymerase chain reaction, temperature, required for the steps A. Denaturation B. Annealing C. Extension
22.DNA gyrase, the enzyme that participates in the process of DNA replication is a type of
23.The most important feature in a plasmid to be used as a vector is
24.The Polymerase Chain Reaction (PCR) is a reaction in which amplification of specific DNA sequences is carried out in vitro. This statement is
25.Protein engineering is used to study the proteins to compare the catalytic properties of:
26.Consider the following statement about PCR I. Polymerase Chain Reaction (PCR) is a technique of synthesizing multiple copies of the desired gene in vitro II. This technique was developed by Kary Mullis in 1985 III. A single PCR amplification cycle involves three basic steps; denaturation, annealing and extension Which of the statement given above are correct?
27.Special sequence in the DNA recognized by restriction endonuclease is called
28.The two main techniques that gave birth to modern biotechnology are I. chemical engineering II. genetic engineering III. human genome engineering IV. molecular biology Choose the correct option
29.Clonal cell lines can be obtained by:
30.The first recombinant DNA was constructed by
31.Name of the drug used in cancer treatment produced by using biotechnology is:
32.Which of the following is correctly matched?
33.The function of polymerase chain reaction (PCR) is:
34.The Ti plasmid used in genetic engineering is obtained from:
35.More advancement in genetic engineering is due to
36.In bacteria, genes for antibiotic resistance are usually located in:
37.Consider the following statements I. In microinjection method foreign DNA is directly injected into the nucleus of animal cell or plant cell by using micro needles or micro pipettes II. Microinjection method is used in oocytes, eggs and embryo III. Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride IV. In chemical mediated gene transfer method certain chemicals such as CO_2 help foreign DNA to enter the host cell Which of the statements given above are correct?
38.Restriction enzymes are isolated chiefly from:
39.DNA can be introduced into any cell by:
40.The restriction enzyme responsible for the cleavage of following sequence is $5'-G-T-C-G-A-C-3'$ $3'-C-A-G-C-T-G-5'$
41.c-DNA probes are copied from the messenger RNA molecules with the help of:
42.Producing a ‘giant mouse’ in the laboratory was possible through:
43.First biochemical to be produced commercially by microbial cloning and genetic engineering is:
44.I. Bacteriophages are _A_ nfectecting _B_ . II. _C_ are hybrid vectors derived from plasmids which contain or site of λ phage A, B and C in above statements refers to
45.Polymerase Chain Reaction (PCR) needs
46.RNA is removed by the treatment with
47.Cutting of a piece of DNA from a plasmid was done with the help of _A_ enzymes, popularly known as _B_ . Here A and B can be
48.Primers are
49.The message from nuclear DNA for the synthesis of specific cytoplasmic protein is carried by:
50.Totipotency in cell is